Product description

Size

2 plate kit

Product code

BEK-2233-2P

Human LR³ insulin-like Growth Factor-I (LR³IGF-I) was developed by GroPep Bioreagents specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin. Supplementation of cell cultures with LR³IGF-I at a much lower concentration results in equivalent or better productivity than supplementation with standard concentrations of insulin. LR³IGF-I is better able to stimulate the type I IGF receptor and thus induce a higher level of activation of intracellular signalling molecules, which are responsible for promoting cell survival by inhibition of apoptosis.This LR³IGF-I Rapid™ ELISA kit combines GroPep’s expertise in the field of IGF research and Biosensis’ newly established Rapid™ ELISA platform. This has resulted in the new LR³IGF-I Rapid™ ELISA kit, which provides for the sensitive, specific and reliable quantification of LR³IGF-I in less than 3 hours. The ELISA kit consists of a complete set of reagents and pre-coated plate to allow immediate assay of LR³IGF-I in culture media. Included in the kit are mouse monoclonal anti- LR³IGF-I capture antibody pre-coated onto an ELISA plate, standard LR³IGF-I protein, a biotinylated anti- LR³IGF-I detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of LR³IGF-I present in samples and the supplied protein standard. This LR³IGF-I Rapid™ ELISA kit has been developed, optimized and validated to quantify LR³IGF-I in cell culture medium. It is likely to be used to measure LR³IGF-I in media and during downstream processing of media following a production cycle and is not intended for other use. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

THE ASSAY SHOULD BEVALIDATEDFOR SPECIFICPURPOSE IN THE USER LABORATORY

CONTACTsales_US@biosensis.comTO PLACE AN ORDER

Fact sheets

LR3 IGF-I Rapid ELISA 2 Plate (Data Sheet)

BEK-2233-2PProtocol

自1990年以来，GroPep Bioreagents便已向大学，研究机构，生物技术和制药公司的研究人员开发，制造和销售生长因子，抗体和相关蛋白试剂。

我们的任务是在准确和可靠的技术建议的支持下，为科学家提供高质量的试剂。

我们科学人员的强大研究背景和经验是我们为客户提供“动手”建议的关键。

我们了解我们的产品并倾听您的需求。

我们通过会议赞助和向会员提供折扣来支持IGF协会